PUMA antibody (C-Term)

Catalog No. ABIN1108799

Product Details anti-PUMA Antibody

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects PUMA (C-term). No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA .Remarks: Sequence is identical to the related rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA (BBC3 Products)

Synonyms: BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody

Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family.1, 2 The PUMA gene is located at 19q.3 PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero-or homodimers, and they act as anti-or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation.4 Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.5Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

Gene ID: 27113

NCBI Accession: NP_001120712

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity