c-MYC antibody (C-Term) (FITC)

Catalog No. ABIN119682

The FITC-conjugated Mouse Monoclonal anti-c-MYC antibody (Clone 9-00E-010) (ABIN119682) specifically detects c-MYC in IF, FACS and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for c-MYC antibody (C-Term) (FITC) (ABIN119682)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is conjugated to FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 9-00E-010

Product Details anti-c-MYC Antibody (FITC)

Binding Specificity: C-Term

Sequence: EQKLISEEDL

Specificity: This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. Membranepermeabilisation is required for this application. Immunoflourescence. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

Gene ID: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect