alpha Adaptin antibody (AA 706-727)
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- Target See all alpha Adaptin (AP2A1) Antibodies
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
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Binding Specificity
- AA 706-727
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Reactivity
- Human, Mouse, Dog, Pig, Horse, Rabbit, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This alpha Adaptin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-ELEPPAPESPMALLADPAPAAD from an internal region of human AP2A1 / AP2-Alpha (NP_055018.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Ferret, Dog, Cat, Panda, Horse, Rabbit, Pig (100%), Rat (95%), Opossum, Guinea pig (91%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product AP2A1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 μg/mL. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
- Alternative Name
- AP2A1 / AP2-Alpha (AP2A1 Products)
- Synonyms
- ADTAA antibody, AP2-ALPHA antibody, CLAPA1 antibody, adtaa antibody, clapa1 antibody, ap2-alpha antibody, AP2A1 antibody, Adtaa antibody, adaptor related protein complex 2 alpha 1 subunit antibody, adaptor-related protein complex 2, alpha 1 subunit antibody, AP-2 complex subunit alpha-1 antibody, AP2A1 antibody, ap2a1 antibody, Ap2a1 antibody, EDI_093420 antibody, EDI_259990 antibody
- Background
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Name/Gene ID: AP2A1
Synonyms: AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha - Gene ID
- 160
- NCBI Accession
- NP_055018
- UniProt
- O95782
- Pathways
- Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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