HIF1A antibody (Biotin)

Catalog No. ABIN1819141

Product Details anti-HIF1A Antibody (Biotin)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIF1A antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human HIF1 alpha (HIF1A), otherwise known as Hypoxia-inducible factor 1-alpha, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to oxygen deprivation (hypoxia). HIF1A and HIF1B (HIF1 beta/ARNT) form the highly-conserved heterodimeric HIF-1 transcriptional complex. During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as vascular endothelial growth factor (VEGF) and glucose transporters. Under normal oxygen conditions, HIF1A is targeted by HIF prolyl-hydroxylase, followed by rapid protease degradation, which is inhibited during hypoxia. Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas. Clone ESEE122 has been shown to be suitable for the staining of snap-frozen cell pellets, see for details.

Purification: Protein G purified

Immunogen: Recombinant human HIF1 alpha.

Type of Immunogen: Recombinant protein

Clone: ESEE122

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1A / HIF1 alpha (HIF1A Products)

Synonyms: hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody

Background: Name/Gene ID: HIF1A

Synonyms: HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1

Gene ID: 3091

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect