AKT3 antibody (AA 119-133)
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- Target See all AKT3 Antibodies
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
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Binding Specificity
- AA 119-133
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This AKT3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- AKT3
- Sequence
- CSPTSQIDNI GEEEM
- Specificity
- This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1, NP_001193658.1).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1, NP_859029.1.
- Isotype
- IgG
- Top Product
- Discover our top product AKT3 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Prostate and Heart. Recommended concentration: 5 μg/mL.
Western Blot: A 50-55 kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0 kDa according to NP_859029.1). Recommended concentration: 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- Alternative Name
- AKT3 (AKT3 Products)
- Synonyms
- MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody
- Background
- AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase, PKB-GAMMA
- Gene ID
- 10000
- NCBI Accession
- NP_005456, NP_859029
- Pathways
- PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling
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