E-cadherin antibody

Catalog No. ABIN1882222

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged CDH1 protein was used to produced this monoclonal antibody.

Clone: 813CT11-1-3

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:4000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-E-cadherin antibody (ABIN1882222)

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." in: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

Ito, Okamoto, Araki, Kawano, Nakao, Fujiyama, Tomita, Mimori, Saya: "Calcium influx triggers the sequential proteolysis of extracellular and cytoplasmic domains of E-cadherin, leading to loss of beta-catenin from cell-cell contacts." in: Oncogene, Vol. 18, Issue 50, pp. 7080-90, (2000) (PubMed).

Oda, Kanai, Oyama, Yoshiura, Shimoyama, Birchmeier, Sugimura, Hirohashi: "E-cadherin gene mutations in human gastric carcinoma cell lines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 5, pp. 1858-62, (1994) (PubMed).

Rimm, Morrow: "Molecular cloning of human E-cadherin suggests a novel subdivision of the cadherin superfamily." in: Biochemical and biophysical research communications, Vol. 200, Issue 3, pp. 1754-61, (1994) (PubMed).

Bussemakers, Giroldi, van Bokhoven, Schalken: "Transcriptional regulation of the human E-cadherin gene in human prostate cancer cell lines: characterization of the human E-cadherin gene promoter." in: Biochemical and biophysical research communications, Vol. 203, Issue 2, pp. 1284-90, (1994) (PubMed).

Bussemakers, van Bokhoven, Mees, Kemler, Schalken: "Molecular cloning and characterization of the human E-cadherin cDNA." in: Molecular biology reports, Vol. 17, Issue 2, pp. 123-8, (1993) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Molecular Weight: 97456

NCBI Accession: NP_004351

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation