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CD22 antibody

CD22 Reactivity: Human, Primate FACS, WB, IP, Inhibition Host: Mouse Monoclonal MEM-01 unconjugated
Catalog No. ABIN206987
  • Target See all CD22 Antibodies
    CD22 (CD22 Molecule (CD22))
    Reactivity
    • 147
    • 48
    • 24
    • 5
    • 3
    • 2
    • 1
    • 1
    Human, Primate
    Host
    • 95
    • 61
    • 13
    Mouse
    Clonality
    • 117
    • 54
    Monoclonal
    Conjugate
    • 79
    • 14
    • 12
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD22 antibody is un-conjugated
    Application
    • 106
    • 68
    • 44
    • 37
    • 37
    • 15
    • 12
    • 11
    • 11
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Antibody (Inhibition)
    Specificity
    Reacts with CD22 (BL-CAM), a 130 kD type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
    Purification
    Protein A purified
    Immunogen
    Raji Burkitt's lymphoma cell line.

    Type of Immunogen: Cells
    Clone
    MEM-01
    Isotype
    IgG1
    Top Product
    Discover our top product CD22 Primary Antibody
  • Application Notes
    Approved: Flo, Inhb, IP, WB (2 - 4 μg/mL)

    Usage: Flow Cytometry: Recommended dilution - 2-4 μg/mL. Western Blotting: Recommended dilution - 2-4 μg/mL, 120 min on vertical incubator. Positive control - RAJI human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis b

    Not recommended for: IHC-P
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, 15 mM sodium azide, approx,  pH 7.4.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C,-20 °C
    Storage Comment
    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target
    CD22 (CD22 Molecule (CD22))
    Alternative Name
    CD22 (CD22 Products)
    Synonyms
    A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
    Background
    Name/Gene ID: CD22
    Family: Immunoglobulin

    Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM
    Gene ID
    933
    UniProt
    P20273
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