H2AFX antibody (pSer139)
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- Target See all H2AFX Antibodies
- H2AFX (H2A Histone Family, Member X (H2AFX))
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Binding Specificity
- AA 134-142, pSer139
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Reactivity
- Human, Cow, Dog, Rabbit, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This H2AFX antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.
- Purification
- Protein G purified
- Immunogen
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Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG1
- Top Product
- Discover our top product H2AFX Primary Antibody
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- Application Notes
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Approved: ICC (2 μg/mL), IF, IP, WB
Usage: Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 μg/mL detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.6, 0.07 % sodium azide, 30 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- H2AFX (H2A Histone Family, Member X (H2AFX))
- Alternative Name
- H2AX (H2AFX Products)
- Synonyms
- H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
- Background
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Name/Gene ID: H2AFX
Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone - Gene ID
- 3014
- UniProt
- P16104
- Pathways
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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