STAT5B antibody (AA 774-787)
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- Target See all STAT5B Antibodies
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
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Binding Specificity
- AA 774-787
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Horse, Pig, Hamster
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This STAT5B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).
- Purification
- Protein A purified
- Immunogen
- Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).
- Isotype
- IgG
- Top Product
- Discover our top product STAT5B Primary Antibody
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- Application Notes
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Approved: IP, WB
Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
- Alternative Name
- STAT5B / STAT (STAT5B Products)
- Synonyms
- STAT5 antibody, stat5 antibody, STAT5A antibody, STAT5B antibody, signal transducer and activator of transcription 5B antibody, signal transducer and activator of transcription 5B S homeolog antibody, Signal transducer and activator of transcription 5B antibody, STAT5B antibody, Stat5b antibody, stat5b.S antibody, LOC490969 antibody, sta5b antibody, LOC100566752 antibody, LOC100052954 antibody
- Background
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Name/Gene ID: STAT5B
Synonyms: STAT5B, Transcription factor STAT5B, STAT5 - Gene ID
- 6777
- UniProt
- P51692
- Pathways
- JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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