PDK4 antibody (AA 117-387)
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- Target See all PDK4 Antibodies
- PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
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Binding Specificity
- AA 117-387
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PDK4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Pyruvate Dehydrogenase Kinase 4 (PDK4)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against PDK4. It has been selected for its ability to recognize PDK4 in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Pyruvate Dehydrogenase Kinase 4 (PDK4) corresdonding to Val117~Gln387 (Accession # O70571)
- Isotype
- IgG
- Top Product
- Discover our top product PDK4 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
- Alternative Name
- Pyruvate Dehydrogenase Kinase 4 (PDK4 Products)
- Synonyms
- AV005916 antibody, pyruvate dehydrogenase kinase 4 antibody, pyruvate dehydrogenase kinase, isoenzyme 4 antibody, pdk4 antibody, PDK4 antibody, Pdk4 antibody
- Background
- [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate Dehydrogenase Kinase Isozyme 4
- Pathways
- PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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