c-MET antibody (pTyr1234, Tyr1235)
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- Target See all c-MET (MET) Antibodies
- c-MET (MET) (Met Proto-Oncogene (MET))
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Binding Specificity
- pTyr1234, Tyr1235
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This c-MET antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- Detects Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody cross-reacts slightly with activated PDGF, insulin and FGF receptors, but it does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human, mouse and monkey. Species sequence homology: rat 100 % .
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.
Type of Immunogen: Synthetic phospho-peptide - Isotype
- IgG
- Top Product
- Discover our top product MET Primary Antibody
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- Application Notes
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Approved: ELISA, ICC (1:50), IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- c-MET (MET) (Met Proto-Oncogene (MET))
- Alternative Name
- c-Met (MET Products)
- Synonyms
- AUTS9 antibody, HGFR antibody, RCCP2 antibody, c-Met antibody, AI838057 antibody, HGF antibody, Par4 antibody, Hgfr antibody, c-met antibody, MET antibody, C-MET antibody, met antibody, met-A antibody, MET proto-oncogene, receptor tyrosine kinase antibody, met proto-oncogene antibody, MET proto-oncogene, receptor tyrosine kinase L homeolog antibody, MET antibody, Met antibody, met.L antibody
- Background
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Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met - Gene ID
- 4233
- UniProt
- P08581
- Pathways
- RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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