Abeta
Reactivity: Human
WB, ELISA, ISt
Host: Mouse
Monoclonal
bmAbetaN
Biotin
Application Notes
Immunoblotting: 1 µg/mL HRP/ECL detection. Recommended blocking with casein/tween 20 based buffer. Western blot Analysis Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2- g/mL Q67350M for 1 hour at 15-22-C and developed by ECL (exp. time: 30 sec.). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43) Immunocytochemistry: 1-10 µg/mL ELISA: 0.1 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute with 1 mL water (15 minutes at room temperature)
Buffer
Lyophilized from 2X PBS, PEG and Sucrose, 0.09 % Sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C/-80 °C
Storage Comment
Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored (up to 3 months) at 2-0 °C.