PIAS2 antibody (Internal Region)

Catalog No. ABIN2466111

Product Details anti-PIAS2 Antibody

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.

Purification: PIAS2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: PIAS2 antibody was raised against a 15 amino acid synthetic peptide near the internal region of PIAS2.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 90 kDa band in lysates of cell line A431after 0.5 μg/mL antibody staining. Immunohistochemistry: In paraffin embedded human testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2 (PIAS2 Products)

Synonyms: MGC83751 antibody, piasx antibody, wu:fi05f09 antibody, PIAS2 antibody, 6330408K17Rik antibody, AI462206 antibody, ARIP3 antibody, AU018068 antibody, Dib antibody, Miz1 antibody, PIASxalpha antibody, PIASxb antibody, PIASxbeta antibody, SIZ2 antibody, DIP antibody, MIZ antibody, MIZ1 antibody, PIASX antibody, PIASX-ALPHA antibody, PIASX-BETA antibody, ZMIZ4 antibody, protein inhibitor of activated STAT 2 L homeolog antibody, protein inhibitor of activated STAT 2 antibody, protein inhibitor of activated STAT, 2 antibody, pias2.L antibody, PIAS2 antibody, pias2 antibody, Pias2 antibody

Gene ID: 9063

NCBI Accession: NP_775298, NP_004662

UniProt: O75928

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling