PIK3CA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PIK3CA antibody was raised against a 14 amino acid synthetic peptide near the internal region of PIK3CA.
PIK3CA
Reactivity: Human
IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/mL). Immunohistochemistry: In paraffin embedded human cerebral cortex shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.