Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Purification
Delipidation and Defibrination
Immunogen
Synthetic peptide corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end.
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
AKT (AKT1/AKT2/AKT3) Antibody can be used for ELISA starting at 1:2000 - 1:10000, Immunofluorescence starting at 1:100 - 1:1000, Immunohistochemistry in paraffin embeded tissues starting at 1:100, Immunoprecipitation, and Western Blotting starting at 1:500 - 1:2000.
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid freeze thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Antibody may be stored short term at 4 °C, long term aliquot and store at -20 °C.