PAK1 antibody (pSer204)

Catalog No. ABIN2568413

Product Details anti-PAK1 Antibody

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Binding Specificity: pSer204

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: PAK1 (phospho Ser204) antibody was raised against a peptide sequence around phosphorylation site of Serine 204 (T-R-S (p)-V-I) derived from Human PAK1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1 (PAK1 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody

Background: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Molecular Weight: 65 kDa

Gene ID: 5058

NCBI Accession: NP_002567

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis