Caspase 8 antibody

Catalog No. ABIN271875

Product Details anti-Caspase 8 Antibody

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Caspase 8 protein. (region surrounding Thr341)

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8 (CASP8 Products)

Synonyms: casp8-A antibody, xCaspase-8 antibody, casp8 antibody, CASP8 antibody, CASP-8 antibody, FLICE antibody, MACH antibody, Mch5 antibody, ALPS2B antibody, CAP4 antibody, Casp-8 antibody, MCH5 antibody, caspase-8 antibody, zgc:92075 antibody, caspase 8 L homeolog antibody, death related ced-3/Nedd2-like protein antibody, caspase 8 antibody, caspase-8 antibody, caspase-8-like antibody, caspase 8, apoptosis-related cysteine peptidase antibody, casp8.L antibody, LOC661095 antibody, CASP8 antibody, LOC5563818 antibody, casp8 antibody, LOC100222284 antibody, Casp8 antibody

Background: Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis, initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog

Molecular Weight: approx. 38, 55 kDa

Gene ID: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades