EIF2AK2 antibody (pThr451)

Catalog No. ABIN2964740

Product Details anti-EIF2AK2 Antibody

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Binding Specificity: pThr451

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Phosphorylation site-specific antibody selective for the phosphorylated form of the PKR (double-stranded RNA-dependent protein kinase) protein containing a phosphate on threonine451. Recognizes a protein of 65-68kD resolved by SDS-PAGE, identified as PKR. Species cross-reactivity: Human and mouse (73 % homologous).

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide derived from the region of PKR that contains threonine451. The sequence was aligned to human.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Positive control: HeLa cells stimulated with interferon gamma (IFN ) and calyculin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Dulbecco's PBS (without Mg2+, Ca2+),  pH 7.3, 50 % glycerol, 1 mg/mL BSA (IgG, protease free), 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: EIF2AK2 / PKR (EIF2AK2 Products)

Synonyms: PKR antibody, pkr antibody, EIF2AK2 antibody, DKFZp469K1934 antibody, EIF2AK1 antibody, PRKR antibody, 2310047A08Rik antibody, 4732414G15Rik antibody, AI467567 antibody, AI747578 antibody, Pkr antibody, Prkr antibody, Tik antibody, eukaryotic translation initiation factor 2 alpha kinase 2 antibody, eukaryotic translation initiation factor 2-alpha kinase 2 antibody, EIF2AK2 antibody, eif2ak2 antibody, Eif2ak2 antibody

Background: Name/Gene ID: EIF2AK2
Subfamily: Translational Control Kinase
Family: Protein Kinase

Synonyms: EIF2AK2, p1/eIF-2A protein kinase, p68 kinase, PRKR, EIF-2A protein kinase 2, p68, Protein kinase RNA-activated, Tik

Gene ID: 5610

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C