HLA-DMB antibody (Middle Region)

Catalog No. ABIN3029729

Product Details anti-HLA-DMB Antibody

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human HLA-DM beta chain (NQKDTLMQRLRNGLQNCATH) was used as the immunogen for this HLA-DM antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the HLA-DM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the HLA-DM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: HLA-DM (Beta Chain) (HLA-DMB Products)

Synonyms: RING7 antibody, D6S221E antibody, RT1.DMb antibody, RT1.Mb antibody, MHC-DMB antibody, major histocompatibility complex, class II, DM beta antibody, RT1 class II, locus DMb antibody, HLA-DMB antibody, RT1-DMb antibody, PATR-DMB antibody

Background: Major histocompatibility complex, class II, DM beta, also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLADMB gene. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chains, both anchored in the membrane. It is located in intracellular vesicles. The protein plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.

UniProt: P28068

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response