FAK antibody (C-Term)

Catalog No. ABIN3043160

Product Details anti-FAK Antibody

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Binding Specificity: AA 1036-1052, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Focal adhesion kinase 1(PTK2) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DVIDQARLKM LGQTRPH

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Focal adhesion kinase 1(PTK2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: PTK2 protein tyrosine kinase 2
Protein Name: Focal adhesion kinase 1(FADK 1)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FAK(1036-1052aa DVIDQARLKMLGQTRPH), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-FAK antibody (ABIN3043160)

Su, Zhang, Mao, Liu, Zheng, Zhang, Su: "Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer." in: Oncology letters, Vol. 9, Issue 2, pp. 595-603, (2015) (PubMed).

Cao, Xing, Yang, Mei: "Correlation between matrix metalloproteinase expression and activation of the focal adhesion kinase signaling pathway in herpes stromal keratitis." in: Experimental and therapeutic medicine, Vol. 7, Issue 1, pp. 280-286, (2013) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: PTK2 (PTK2 Products)

Synonyms: FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

Background: PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Synonyms: FADK 1 antibody|FADK antibody|FAK 1 antibody|FAK related non kinase polypeptide antibody|FAK1 antibody|FAK1_HUMAN antibody|Focal adhesion kinase 1 antibody|Focal adhesion kinase related nonkinase antibody|FRNK antibody|p125FAK antibody|pp125FAK antibody|PPP1R71 antibody|Protein phosphatase 1 regulatory subunit 71 antibody|Protein tyrosine kinase 2 antibody|Protein Tyrosine Kinase Cytoplasmic antibody|Protein-tyrosine kinase 2 antibody|PTK 2 antibody|PTK2 antibody|PTK2 protein tyrosine kinase 2 antibody

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling