Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (AA 240-259)

Details for Product anti-CASP8 Antibody No. ABIN3044361
Antigen
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8 L homeolog
  • death related ced-3/Nedd2-like protein
  • caspase 8
  • caspase-8
  • caspase-8-like
  • caspase 8, apoptosis-related cysteine peptidase
  • casp8.L
  • LOC661095
  • CASP8
  • LOC5563818
  • casp8
  • LOC100222284
  • Casp8
Alternatives
anti-Human Caspase 8 antibody for ELISA
Epitope
AA 240-259, Middle Region
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Reactivity
Human, Rat (Rattus)
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133
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This Caspase 8 antibody is un-conjugated
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Application
Western Blotting (WB)
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Options
Purpose Rabbit IgG polyclonal antibody for Caspase-8(CASP8) detection. Tested with WB in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8(P18)(240-259aa DKVYQMKSKPRGYCLIINNH), different from the related mouse sequence by one amino acid.
Sequence DKVYQMKSKP RGYCLIINNH
Isotype IgG
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Caspase-8(CASP8) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: caspase 8, apoptosis-related cysteine peptidase
Protein Name: Caspase-8(CASP-8)
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others Caspase 8 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CASP8 (CASP8 Antibody Abstract)
Background Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease(caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme(ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex(DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.

Synonyms: ALPS2B antibody|Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody|Apoptosis related cysteine peptidase antibody|Apoptotic cysteine protease antibody|Apoptotic protease Mch 5 antibody|Apoptotic protease Mch-5 antibody|Apoptotic protease Mch5 antibody|CAP 4 antibody|CAP4 antibody|CASP 8 antibody|CASP-8 antibody|CASP8 antibody|Caspase 8 antibody|Caspase 8 apoptosis related cysteine peptidase antibody|Caspase-8 subunit p18 antibody|Caspase8 antibody|CED 3 antibody|FADD homologous ICE/CED 3 like protease antibody|FADD Homologous ICE/CED3 Like Protease antibody|FADD Like ICE antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|FLJ17672 antibody|ICE like apoptotic protease 5 antibody|ICE-like apoptotic protease 5 antibody|MACH alpha 1/2/3 protein antibody|MACH antibody|MACH beta 1/2/3/4 protein antibody|MCH 5 antibody|MCH5 antibody|MGC78473 antibody|MORT1 associated CED 3 homolog antibody|MORT1 associated CED3 homolog antibody|MORT1-associated CED-3 homolog antibody|OTTHUMP00000163717 antibody|OTTHUMP00000163720 antibody|OTTHUMP00000163724 antibody|OTTHUMP00000163725 antibody|OTTHUMP00000165062 antibody|OTTHUMP00000165063 antibody|OTTHUMP00000165064 antibody|OTTHUMP00000206552 antibody|OTTHUMP00000206582 antibody
UniProt Q14790
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 240-259), (Middle Region) antibody (ABIN3044361) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 240-259), (Middle Region) antibody
Product cited in: Zhan, Hu, Yi, An, Huang: "[Corrigendum] Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 8010, 2018 (PubMed).

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, 2017 (PubMed).

Zhang, Huang, Wang, Luo, Wang: "2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3442-8, 2016 (PubMed).

Li, Wu, Sun, Zhao, Liu, Zhang: "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 17, Issue 11, pp. 4893-4898, 2016 (PubMed).

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, 2015 (PubMed).

Liu, Chen, Wang, Yang, Xue, Zhu: "Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells." in: FEBS letters, Vol. 589, Issue 8, pp. 897-903, 2015 (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014 (PubMed).

Yan, Liang, Zhang, Liu, Bu: "Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL-RVG) in vitro." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 317-26, 2014 (PubMed).

Shan, Li, Newton, Zhao, Li, Guo: "A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells." in: Toxicology letters, Vol. 227, Issue 2, pp. 129-38, 2014 (PubMed).

Xia, Luo, Lin, Zhang, Guo, He: "The effect of different treatment time of millimeter wave on chondrocyte apoptosis, caspase-3, caspase-8, and MMP-13 expression in rabbit surgically induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 9, pp. 2847-56, 2012 (PubMed).

Lin, He, Luo, Zhang, Zeng: "The effect of low-level laser to apoptosis of chondrocyte and caspases expression, including caspase-8 and caspase-3 in rabbit surgery-induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 3, pp. 759-66, 2012 (PubMed).

Zeng, Luo, Lin, Zhang, He: "The effect of therapeutic ultrasound to apoptosis of chondrocyte and caspase-3 and caspase-8 expression in rabbit surgery-induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 12, pp. 3771-7, 2012 (PubMed).

Lian, Ni, Dai, Su, Smith, Xu, He: "Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation." in: Molecular cancer therapeutics, Vol. 11, Issue 2, pp. 416-26, 2012 (PubMed).

Guo, Luo, Zhang, Lin, Xia, He: "Comparing different physical factors on serum TNF-? levels, chondrocyte apoptosis, caspase-3 and caspase-8 expression in osteoarthritis of the knee in rabbits." in: Joint, bone, spine : revue du rhumatisme, Vol. 78, Issue 6, pp. 604-10, 2011 (PubMed).

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