GZMB antibody
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- Target See all GZMB Antibodies
- GZMB (Granzyme B (GZMB))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GZMB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- Specificity
- Recognizes human Granzyme B. Shows no cross-reactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.
- Purification
- Protein G purified
- Immunogen
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NS0-derived rhGranzyme B.
Type of Immunogen: Recombinant protein - Clone
- 351927
- Isotype
- IgG2a
- Top Product
- Discover our top product GZMB Primary Antibody
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- Application Notes
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Approved: ELISA, Flo, IHC, WB
Usage: Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human Granzyme B. Using a colorimetric detection system, the detection limit for rhGranzyme B is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was tested for flow cytometry using NK-92 cells. For intracellular staining to detect human Granzyme B, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Immunohistochemistry: This antibody was used at a concentration of 8-25 μg/mL with appropriate secondary reagents to detect human Granzyme B in paraformaldehyde-fixed human PBMC. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Granzyme B. The detection limit for rhGranzyme B is approximately 5 ng/well. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- Target
- GZMB (Granzyme B (GZMB))
- Alternative Name
- GZMB / Granzyme B (GZMB Products)
- Synonyms
- CCPI antibody, CGL-1 antibody, CGL1 antibody, CSP-B antibody, CSPB antibody, CTLA1 antibody, CTSGL1 antibody, HLP antibody, SECT antibody, granzyme B antibody, GZMB antibody
- Background
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Name/Gene ID: GZMB
Subfamily: Serine S1
Family: Protease
Synonyms: GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2 - Gene ID
- 3002
- UniProt
- P10144
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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