PIAS2 antibody (AA 431-445)

Catalog No. ABIN461934

Product Details anti-PIAS2 Antibody

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Binding Specificity: AA 431-445

Reactivity: Human, Dog, Horse, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PIAS2. This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bat, Horse, Rabbit (100%) Marmoset, Hamster, Elephant (93%) Mouse, Rat, Bovine (87%) Platypus (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KEAMKVSSQPCTKIE from an internal region of human PIAS2 (NP_775298.1, NP_004662.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bat, Horse, Rabbit (100%), Marmoset, Hamster, Elephant (93%), Mouse, Rat, Bovine (87%), Platypus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2 / PIASX (PIAS2 Products)

Synonyms: MGC83751 antibody, piasx antibody, wu:fi05f09 antibody, PIAS2 antibody, 6330408K17Rik antibody, AI462206 antibody, ARIP3 antibody, AU018068 antibody, Dib antibody, Miz1 antibody, PIASxalpha antibody, PIASxb antibody, PIASxbeta antibody, SIZ2 antibody, DIP antibody, MIZ antibody, MIZ1 antibody, PIASX antibody, PIASX-ALPHA antibody, PIASX-BETA antibody, ZMIZ4 antibody, protein inhibitor of activated STAT 2 L homeolog antibody, protein inhibitor of activated STAT 2 antibody, protein inhibitor of activated STAT, 2 antibody, pias2.L antibody, PIAS2 antibody, pias2 antibody, Pias2 antibody

Background: Name/Gene ID: PIAS2

Synonyms: PIAS2, ARIP3, DAB2-interacting protein, DIP, MIZ, MIZ1, PIAS-NY protein, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, E3 SUMO-protein ligase PIAS2

Gene ID: 9063

NCBI Accession: NP_775298, NP_004662

UniProt: O75928

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling