H2AFX antibody (N-Term)
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- Target See all H2AFX Antibodies
- H2AFX (H2A Histone Family, Member X (H2AFX))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Monkey, Guinea Pig, Hamster, Zebrafish (Danio rerio), Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This H2AFX antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Human H2AFX / H2AX
- Predicted Reactivity
- Percent identity by BLAST analysis: Rat, Bovine, Zebrafish (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human H2AFX (P16104, NP_002096). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig, Platypus, Trout, Salmon, Pike, Zebrafish (100%), Elephant, Panda, Turkey, Zebra finch, Chicken, Xenopus (92%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product H2AFX Primary Antibody
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- Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- H2AFX (H2A Histone Family, Member X (H2AFX))
- Alternative Name
- H2AX (H2AFX Products)
- Synonyms
- H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
- Background
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Name/Gene ID: H2AFX
Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone - Gene ID
- 3014
- NCBI Accession
- NP_002096
- UniProt
- P16104
- Pathways
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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