IGF1 antibody (Biotin)

Catalog No. ABIN465004

Product Details anti-IGF1 Antibody (Biotin)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human IGF-I

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-I.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hIGF-I by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. To detect hIGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-I (LS-B7414) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-I / IGF1 (IGF1 Products)

Synonyms: IGF-I antibody, IGF1A antibody, IGFI antibody, C730016P09Rik antibody, Igf-1 antibody, Igf-I antibody, Npt2B antibody, IGF-1 antibody, IGFIA antibody, IGF-IB antibody, igf1 antibody, IGF-1L antibody, IGF-1a antibody, IGF1 antibody, igf-1 antibody, igf1-A antibody, igf1.S antibody, xigf1 antibody, insulin like growth factor 1 antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I antibody, insulin like growth factor 1 L homeolog antibody, IGF1 antibody, Igf1 antibody, LOC100136741 antibody, igf1 antibody, LOC678666 antibody, igf1.L antibody, LOC104911603 antibody

Background: Name/Gene ID: IGF1
Family: Hormone

Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF

Gene ID: 3479

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation