PAI1 antibody (Biotin)

Catalog No. ABIN465370

Product Details anti-PAI1 Antibody (Biotin)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAI1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human PAI-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human PAI-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hPAI-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. To detect hPAI-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PAI-1 (LS-B7483) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1 / PAI-1 (SERPINE1 Products)

Synonyms: PAI antibody, PAI-1 antibody, PAI1 antibody, PLANH1 antibody, SERPINE1 antibody, si:ch211-138a11.1 antibody, Planh1 antibody, PAI1A antibody, Pai1 antibody, Pai1aa antibody, Planh antibody, RATPAI1A antibody, serpin family E member 1 antibody, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog antibody, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 antibody, serine (or cysteine) peptidase inhibitor, clade E, member 1 antibody, SERPINE1 antibody, serpine1.L antibody, serpine1 antibody, Serpine1 antibody

Background: Name/Gene ID: SERPINE1
Family: Serine Protease Inhibitor

Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1

Gene ID: 5054

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration