PLCG1
Reactivity: Mouse, Rat
WB, ELISA, IF (p), IHC (p), IF (cc), FACS, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
Positive Control Provided. Cell lysate from untreated SKOV-3 cellsDescription: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL H2O (15 min, RT).
Buffer
1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date
12 months
Target
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))
Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: EC=3.1.4.11, PLC-148, PLC-II, PLC-gamma, PLC-gamma-1, PLC1, PLCG-1, Phosphoinositide Phospholipase C, Phospholipase C gamma, Phospholipase C-gamma-1