c-MYC antibody (pThr58)

Catalog No. ABIN3029537

The Rabbit Polyclonal anti-c-MYC antibody is suitable to detect c-MYC in samples from Human. It has been validated for WB, ELISA and DB.

Quick Overview for c-MYC antibody (pThr58) (ABIN3029537)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-c-MYC Antibody

Binding Specificity: pThr58

Cross-Reactivity (Details): Expected species reactivity: Bovine, Chicken, Mouse, Pig, Rat, Xenopus, Zebrafish

Purification: Antigen affinity purified

Immunogen: This phospho c-Myc antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT58 of human MYC.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho c-Myc antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho c-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: C-Myc participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect