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GRB2 antibody
GRB2
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-GRB2 Antibody
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Target
See all GRB2 Antibodies
GRB2
(Growth Factor Receptor-Bound Protein 2 (GRB2))
Reactivity
All reactivities for GRB2 antibodies
Human, Mouse, Rat
Host
All hosts for GRB2 antibodies
Rabbit
Clonality
All clonalities for GRB2 antibodies
Polyclonal
Conjugate
All conjugates for GRB2 antibodies
This GRB2 antibody is un-conjugated
Application
All applications for GRB2 antibodies
Western Blotting (WB)
Specificity
This antibody detects endogenous levels of GRB2 protein. (region surrounding Arg178)
Cross-Reactivity (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity chromatography using epitope-specific immunogen
Purity
> 95 % pure by SDS-PAGE
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Discover our top product GRB2 Primary Antibody
Alternatives
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Application Details
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Application Notes
Western blot: 1: 500 - 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target Details for GRB2
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Target
GRB2
(Growth Factor Receptor-Bound Protein 2 (GRB2))
Alternative Name
GRB2 / ASH (GRB2 Products )
Synonyms
GRB2 antibody, grb2 antibody, AA408164 antibody, Ash antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, wu:fb18e12 antibody, wu:fb34e06 antibody, zgc:73077 antibody, growth factor receptor bound protein 2 antibody, growth factor receptor-bound protein 2 antibody, growth factor receptor-bound protein 2b antibody, GRB2 antibody, grb2 antibody, Grb2 antibody, grb2b antibody
Background
Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2
Molecular Weight
approx. 32 kDa
Gene ID
2885
NCBI Accession
NP_002077
UniProt
P62993
Pathways
RTK Signaling , TCR Signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Regulation of Actin Filament Polymerization , Hepatitis C , Signaling Events mediated by VEGFR1 and VEGFR2 , Signaling of Hepatocyte Growth Factor Receptor , EGFR Downregulation
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