MGMT
Reactivity: Human
WB, IF
Host: Mouse
Polyclonal
unconjugated
Application Notes
Flow Cytometry (1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In buffer containing 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,-80 °C
Storage Comment
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Cai, Xu, Cooper, Ferkowicz, Hartwell, Pollok, Kelley: "Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents." in: Cancer research, Vol. 65, Issue 8, pp. 3319-27, (2005) (PubMed).
Zuo, Ai, Ratliff, Suen, Hanna, Brent, Fan: "O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 13, Issue 6, pp. 967-75, (2004) (PubMed).
Roth, Xu, Luo, Kelley: "Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection." in: Cancer gene therapy, Vol. 10, Issue 8, pp. 603-10, (2003) (PubMed).