ELISA (1:3000-1:12000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Stros, Muselíková-Polanská, Pospísilová, Strauss: "High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops." in: Biochemistry, Vol. 43, Issue 22, pp. 7215-25, (2004) (PubMed).
Aslanian, Iaquinta, Verona, Lees: "Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics." in: Genes & development, Vol. 18, Issue 12, pp. 1413-22, (2004) (PubMed).
Pospísilová, Siligan, Ban, Jug, Kovar: "Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y." in: Molecular cancer research : MCR, Vol. 2, Issue 5, pp. 296-304, (2004) (PubMed).
Target
MDM2
(Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))