Synonyms: HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PASprotein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helixprotein 78
Purification
Protein G Chromatography.
Immunogen
Recombinant fragment corresponding to amino acids 329-530.
ELISA. Immunofluorescence/Immunocytochemistry: 8-12 μg/mLImmunohistochemistry: (Frozen/Paraffin) 1/1000-1/8000. Western blot: 1 μg/mL was sufficient for detection of HIF1 alpha in 20 μg of C °Cl2-inducedHela cell lysate by colorimetric immunoblot analysis using Goat anti-Mouse IgG: HRP as the
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF1-alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF1 alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF1-alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).Synonyms: ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1