FOXO3 antibody (C-Term)

Catalog No. ABIN374440

Product Details anti-FOXO3 Antibody

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-GAKQASSQSW VPG

Specificity: This antibody recognizes FKHRL1/FOXO3A.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to according to NP_001446.1. Genename: FOXO3

Application Details

Application Notes: Peptide ELISA: 1/32,000. Western Blot: Approx 90 kDa band observed in human Brain and Heart lysates (calculatedMW of 71.0 kDa according to NP_001446 and NP_963853). Recommended concentration: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3 / FKHRL1 (FOXO3 Products)

Synonyms: AF6q21 antibody, FKHRL1 antibody, FKHRL1P2 antibody, FOXO2 antibody, FOXO3A antibody, zgc:92176 antibody, wu:fb50g02 antibody, wu:fi33f07 antibody, Fkhrl1 antibody, Foxo3a antibody, FoxO3A antibody, 1110048B16Rik antibody, 2010203A17Rik antibody, C76856 antibody, Fkhr2 antibody, FOXO-3 antibody, FOXO3 antibody, foxo3a antibody, xfoxo3 antibody, foxO3 antibody, foxO antibody, fkhr antibody, foxo5 antibody, wu:fj65a12 antibody, zFkhr antibody, forkhead box O3 antibody, forkhead box O3A antibody, forkhead box O3 L homeolog antibody, forkhead box O3b antibody, FOXO3 antibody, foxo3a antibody, Foxo3 antibody, foxo3.L antibody, foxo3 antibody, foxO3 antibody, foxo3b antibody

Background: FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two tiered mechanism of phosphorylation and acetylation. FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevitySynonyms: AF6q21 protein, FOXO3A, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1

Gene ID: 2309

NCBI Accession: NP_001446

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis