Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human GSK3A.
Specificity
GSK3A (phospho S21) polyclonal antibody detects endogenous level of GSK3A only when phosphorylated at serine 21 and does not detect GSK3B when phosphorylated at serine 9.
Cross-Reactivity
Human, Mouse, Rabbit
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human GSK3A.
GSK3a
Reactivity: Human
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunofluorescence (1:100-1:200) Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.