This antibody is not expected to cross-react with the similar P450 1A2
Purification
cytochrome P450 1A1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
cytochrome P450 1A1 Antibody was raised against synthetic peptide CQEKQLDENANVQ found at an internal region of cytochrome P450 1A1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 85-90 kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2 kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
cytochrome P450 1A1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
CYP1A1
(Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))