IGF1 antibody
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- Target See all IGF1 Antibodies
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IGF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Purification
- Anti-hIGF-1 specific antibody was purified by affinity chromatography employing immobilized hIGF-1 matrix.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
- Top Product
- Discover our top product IGF1 Primary Antibody
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- Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-1 (3.00 ng/mL), a concentration of 0.67 0.1 μ,g/mL of this antibody is required.
ELISA:
To detect hIGF-1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-1.
Sandwich:
To detect hIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-I (XP-5158Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
Western Blot:
To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
- Alternative Name
- IGF-I (IGF1 Products)
- Synonyms
- IGF-I antibody, IGF1A antibody, IGFI antibody, C730016P09Rik antibody, Igf-1 antibody, Igf-I antibody, Npt2B antibody, IGF-1 antibody, IGFIA antibody, IGF-IB antibody, igf1 antibody, IGF-1L antibody, IGF-1a antibody, IGF1 antibody, igf-1 antibody, igf1-A antibody, igf1.S antibody, xigf1 antibody, insulin like growth factor 1 antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I antibody, insulin like growth factor 1 L homeolog antibody, IGF1 antibody, Igf1 antibody, LOC100136741 antibody, igf1 antibody, LOC678666 antibody, igf1.L antibody, LOC104911603 antibody
- Gene ID
- 3486
- Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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