Retinoblastoma 1 antibody
-
- Target See all Retinoblastoma 1 (RB1) Antibodies
- Retinoblastoma 1 (RB1)
-
Reactivity
- Human, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Retinoblastoma 1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- Immunogen
- retinoblastoma 1
- Isotype
- IgG
- Top Product
- Discover our top product RB1 Primary Antibody
-
-
- Application Notes
- WB : 1:1000-1:10000 IP : 1:200-1:2000 IHC : 1:50-1:200
- Comment
-
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07141(RB1 antibody) at dilution of 1:3000
- Restrictions
- For Research Use only
-
- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze / thaw cycles.
- Storage
- -20 °C
- Expiry Date
- 12 months
-
- Target
- Retinoblastoma 1 (RB1)
- Alternative Name
- RB1 (RB1 Products)
- Synonyms
- OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
- Background
- Synonyms:OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1 Background:Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase(HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex(By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
- Molecular Weight
- 110kDa
- Gene ID
- 5925
- UniProt
- P06400
- Pathways
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
-