RASSF1 antibody (AA 135-147)

Catalog No. ABIN574096

Product Details anti-RASSF1 Antibody

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Binding Specificity: AA 135-147

Reactivity: Human, Cow, Horse, Pig, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This RASSF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RASSF1 / RASSF1A. This antibody is expected to recognize reported isoforms A, C and D (NP_009113.3, NP_733831.1, NP_733832.1) .

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Bat, Bovine, Horse, Pig, Lizard (100%) Marmoset, Panda, Dog, Rabbit, Platypus (92%) Mouse, Rat, Hamster, Opossum (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SQAEIEQKIKEYN from an internal region of human RASSF1 / RASSF1A (NP_009113.3, NP_733830.1, NP_733831.1, NP_733832.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Bat, Bovine, Horse, Pig, Lizard (100%), Marmoset, Panda, Dog, Rabbit, Platypus (92%), Mouse, Rat, Hamster, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: RASSF1 / RASSF1A (RASSF1 Products)

Synonyms: RASSF1 antibody, zgc:92505 antibody, wu:fc17f02 antibody, zgc:112224 antibody, 123F2 antibody, NORE2A antibody, RASSF1A antibody, RDA32 antibody, REH3P21 antibody, AA536941 antibody, AU044980 antibody, Rassf1A antibody, Rassf1B antibody, Rassf1C antibody, NPR2-like, GATOR1 complex subunit antibody, Ras association (RalGDS/AF-6) domain family 1 antibody, Ras association domain family member 1 antibody, putative rassf1 antibody, Ras association domain family member 1 L homeolog antibody, Ras association (RalGDS/AF-6) domain family member 1 antibody, NPRL2 antibody, rassf1 antibody, RASSF1 antibody, Smp_157340 antibody, rassf1.L antibody, Rassf1 antibody

Background: Name/Gene ID: RASSF1

Synonyms: RASSF1, 123F2, RASSF1A, RDA32, Tumor suppressor protein RDA32, NORE2A, WUGSC:H_LUCA12.5, REH3P21

Gene ID: 11186

NCBI Accession: NP_009113, NP_733830, NP_733831, NP_733832

UniProt: Q9NS23