FAK antibody (pTyr397)
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- Target See all FAK (PTK2) Antibodies
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
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Binding Specificity
- AA 340-420, pTyr397
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FAK antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- FAK is a protein encoded by the PTK2 gene which is approximately 119,2 kDa. FAK is localised to the cell membrane and cytoplasm. It is involved in ERK signalling, integrin pathway and EPH-ephrin signalling. It is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. FAK is ubiquitously expressed with Isoform 1 and isoform 6 expressed in lung fibroblasts. Mutations in the PTK2 gene may result in malignant glioma. STJ93024 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Y397.This primary antibody specifically binds to endogenous FAK protein which only binds about Y397 when Y397 is phosphorylated.
- Specificity
- Phospho-FAK (Y397) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y397.
- Characteristics
- Rabbit polyclonal to Phospho-FAK (Y397).
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human FAK around the phosphorylation site of Y397.
- Isotype
- IgG
- Top Product
- Discover our top product PTK2 Primary Antibody
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- Application Notes
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000 - Comment
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Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- Alternative Name
- FAK (PTK2 Products)
- Synonyms
- FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody
- Molecular Weight
- 119 kDa
- Gene ID
- 5747
- UniProt
- Q05397
- Pathways
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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