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H2AFX antibody (AA 80-160)
H2AFX
Reactivity: Human, Mouse, Rat
WB, ELISA, IF, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-H2AFX Antibody
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Target
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H2AFX
(H2A Histone Family, Member X (H2AFX))
Binding Specificity
All epitopes for H2AFX antibodies
AA 80-160
Reactivity
All reactivities for H2AFX antibodies
Human, Mouse, Rat
Host
All hosts for H2AFX antibodies
Rabbit
Clonality
All clonalities for H2AFX antibodies
Polyclonal
Conjugate
All conjugates for H2AFX antibodies
This H2AFX antibody is un-conjugated
Application
All applications for H2AFX antibodies
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
Histone H2A.X is a protein encoded by the H2AFX gene which is approximately 15,1 kDa. Histone H2A.X is localised to the nucleus. It is involved in DNA double-strand break repair and mitotic cell cycle. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units. Histone H2A.X is expressed in the lung, nervous system, intestine, skin and eye. Mutations in the H2AFX gene may result in Nijmegen breakage syndrome and ataxia-telangiectasia. STJ93520 was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. This antibody detects endogenous Histone H2A.X.
Specificity
Histone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein.
Characteristics
Rabbit polyclonal to Histone H2A.X.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Synthesized peptide derived from human Histone H2A.X around the non-phosphorylation site of S139.
Isotype
IgG
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Discover our top product H2AFX Primary Antibody
Alternatives
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Application Details
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Application Notes
WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store at -20°C, and avoid repeat freeze-thaw cycles.
Target Details for H2AFX
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Target
H2AFX
(H2A Histone Family, Member X (H2AFX))
Alternative Name
Histone H2A.X (H2AFX Products )
Synonyms
H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
Molecular Weight
19 kDa
Gene ID
3014
UniProt
P16104
Pathways
Telomere Maintenance , DNA Damage Repair , Positive Regulation of Response to DNA Damage Stimulus
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