PPP1R12A antibody (pThr853)
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- Target See all PPP1R12A Antibodies
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
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Binding Specificity
- AA 590-670, pThr853
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPP1R12A antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- MYPT1 is a protein encoded by the PPP1R12A gene which is approximately 115,2 kDa. MYPT1 is localised to the cytoplasm. It is involved in the activation of cAMP-dependent PKA, beta-adrenergic signalling, vascular smooth muscle contraction and actin nucleation by ARP-WASP complex. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho and is also a key regulator of protein phosphatase 1C. MYPT1 is expressed in striated muscles, specifically in type 2a fibres. Mutations in the PPP1R12A gene may result in infratentorial cancer and medullomyoblastoma. STJ90822 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MYPT1 protein which only binds about T853 when T853 is phosphorylated.
- Specificity
- Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.
- Characteristics
- Rabbit polyclonal to Phospho-MYPT1 (T853).
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.
- Isotype
- IgG
- Top Product
- Discover our top product PPP1R12A Primary Antibody
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- Application Notes
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000 - Comment
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Expressed in striated muscles, specifically in type 2a fibers (at protein level).
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- Alternative Name
- MYPT1 (PPP1R12A Products)
- Synonyms
- 1200015F06Rik antibody, 5730577I22Rik antibody, AA792106 antibody, AV099298 antibody, D10Ertd625e antibody, Mypt1 antibody, M130 antibody, MBS antibody, MYPT1 antibody, M110 antibody, MBSP antibody, protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, protein phosphatase 1 regulatory subunit 12A antibody, protein phosphatase 1, regulatory subunit 12A antibody, Ppp1r12a antibody, PPP1R12A antibody
- Molecular Weight
- 130 kDa
- Gene ID
- 4659
- UniProt
- O14974
- Pathways
- M Phase
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