CD22 antibody (AA 52-178)
-
- Target See all CD22 Antibodies
- CD22 (CD22 Molecule (CD22))
-
Binding Specificity
- AA 52-178
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD22 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Purified
- Purity
- Protein G affinity chromatography
- Immunogen
- Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.
- Clone
- BLCAM-1795
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD22 Primary Antibody
-
-
- Application Notes
- Optimal dilution of the CD22 antibody should be determined by the researcher.\. ELISA: 2-4 μg/mL (order BSA free formulation for coating),Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT
- Restrictions
- For Research Use only
-
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD22 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Target
- CD22 (CD22 Molecule (CD22))
- Alternative Name
- CD22 (CD22 Products)
- Synonyms
- A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
- Background
- Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
-