ARAF antibody (pSer214) (HRP)

Catalog No. ABIN682777

Product Details anti-ARAF Antibody (HRP)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Binding Specificity: pSer214

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser214

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: A Raf (ARAF Products)

Synonyms: zgc:92074 antibody, wu:fk45h07 antibody, ARAF antibody, a-raf antibody, araf antibody, araf1 antibody, pks2 antibody, rafa1 antibody, A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, 1200013E08Rik antibody, A-Raf antibody, AW495444 antibody, Araf1 antibody, A-Raf proto-oncogene, serine/threonine kinase antibody, A-Raf proto-oncogene, serine/threonine kinase L homeolog antibody, A-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Araf proto-oncogene, serine/threonine kinase antibody, araf antibody, ARAF antibody, araf.L antibody, araf.S antibody, Araf antibody

Background:

Synonyms: A raf 1, A Raf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, ARaf proto oncogene serine/threonine protein kinase, ARAF1, Oncogene Araf1, Oncogene PKS2, PKS 2, PKS, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, ARAF_HUMAN.

Background: The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.

Gene ID: 369

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C