CD22 antibody (AA 52-178)
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- Target See all CD22 Antibodies
- CD22 (CD22 Molecule (CD22))
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Binding Specificity
- AA 52-178
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD22 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
- Clone
- BLCAM-1795
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD22 Primary Antibody
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- Application Notes
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Raji, Ramos, MOLT4 and human peripheral blood lymphocytes. Tonsil, Lymph Node or Spleen.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CD22 (CD22 Molecule (CD22))
- Alternative Name
- CD22 (CD22 Products)
- Synonyms
- A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
- Background
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B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 / BL-CAM (B-Cell Marker)
Cellular localisation: Cell Surface - Molecular Weight
- 130-140kDa
- Gene ID
- 933, 579691
- UniProt
- P20273
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