GZMB antibody (AA 73-187)

Catalog No. ABIN6937361

Product Details anti-GZMB Antibody

Target: GZMB (Granzyme B (GZMB))

Binding Specificity: AA 73-187

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Cross-Reactivity (Details): Human. Dog

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Clone: GZMB-3014

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Human tonsil, spleen or Hodgkin's Lymphoma tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB (GZMB Products)

Synonyms: CCPI antibody, CGL-1 antibody, CGL1 antibody, CSP-B antibody, CSPB antibody, CTLA1 antibody, CTSGL1 antibody, HLP antibody, SECT antibody, granzyme B antibody, GZMB antibody

Background: Cathepsin G-like 1, CCPI, CGL1, CSPB, CTLA-1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Human lymphocyte protein (Hlp), Lymphocyte protease, SECT, T-cell serine protease 1-3E,Granzyme B (NK/T-Cell Lymphoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 29-32kDa

Gene ID: 3002, 1051

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis