HLA-DR antibody

Catalog No. ABIN6937411

Product Details anti-HLA-DR Antibody

Target: HLA-DR

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC class II/ dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the sub- sequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain ( -Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Clone: TAL 1B5

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji, Ramos or Daudi whole cell lysates. Spleen, tonsil or liver tissue lysates. Human melanoma, tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR (HLA-DR Products)

Background: DR alpha chain, DRB1, DRB4, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility complex class II DR alpha, Major histocompatibility complex class II DR beta 1/3/4/5,HLA-DR (MHC II)
Cellular localisation: Cell surface

Molecular Weight: 36kDa (alpha chain), 27kDa (beta chain), 63kDa (HLA-DR dimer)

Gene ID: 3122, 520048

UniProt: P01903