CDK8 antibody (AA 21-392)

Catalog No. ABIN7441010

Product Details anti-CDK8 Antibody

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Binding Specificity: AA 21-392

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Cyclin Dependent Kinase 8 (CDK8)

Specificity: The antibody is a rabbit polyclonal antibody raised against CDK8. It has been selected for its ability to recognize CDK8 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cyclin Dependent Kinase 8 (CDK8) corresdonding to Glu21~Asp392 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: Cyclin Dependent Kinase 8 (CDK8 Products)

Synonyms: K35 antibody, wu:fk95h11 antibody, cyclin dependent kinase 8 antibody, cyclin-dependent kinase 8 antibody, cyclin-dependent kinase 8 S homeolog antibody, Cyclin-dependent kinase 8 antibody, cyclin dependent kinase 19 antibody, CDK8 antibody, Cdk8 antibody, cdk8 antibody, cdk8.S antibody, cdk-8 antibody, CDK19 antibody

Background: K35, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha