c-MET antibody (pTyr1234, pTyr1235) (Biotin)

Catalog No. ABIN743845

This Rabbit Polyclonal antibody specifically detects c-MET in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for c-MET antibody (pTyr1234, pTyr1235) (Biotin) (ABIN743845)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MET antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-c-MET Antibody (Biotin)

Binding Specificity: pTyr1234, pTyr1235

Specificity: These phosphorylation sites are homologous to Tyr1232 + Ty1233 in Mouse and Tyr1235 + Tyr1236 in Rat. Due to the highly conserved nature of this domain, there is a chance that this antibody will react with MSP receptors in Mouse and, less likely, in Human when phosphorylated at the the sites of Ser1238 and Ser1239 in Human, Ser1215 and Ser1216 in Mouse. No phosphorylation information is currently available Rat.

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyr1234/1235

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: c-Met +

Background:

Synonyms: HGFR, AUTS9, RCCP2, c-Met, Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET

Background: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor