FAK antibody (pTyr397)

Catalog No. ABIN871434

Product Details anti-FAK Antibody

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Binding Specificity: pTyr397

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.

Purification: Affinity purified

Immunogen: Peptide sequence around phosphorylation site of pTyr397 (D-D-Y (p) -A-E) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-FAK antibody (ABIN871434)

Zheng, Wei, Wang, Gong, Zhang: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." in: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, (2009) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK (PTK2 Products)

Synonyms: FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

Background: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Molecular Weight: 125 kDa

Gene ID: 5747

NCBI Accession: NP_001186

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling