c-MYC antibody

Catalog No. ABIN920762

The Chicken Polyclonal anti-c-MYC antibody is suitable to detect c-MYC. It has been validated for WB and ELISA.

Quick Overview for c-MYC antibody (ABIN920762)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Host: Chicken

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-c-MYC Antibody

Purification: Affinity purified

Application Details

Application Notes: Western blot: Use at ~1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 with 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: Chickens were immunized with the C-Myc peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA. A 1:38,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using HRP- conjugated Goat anti-Chicken IgY (IgG) at 1:10,000 and TMB as the substrate. Appropriate specificity controls were run.

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect